Tris(hydroxymethyl)aminomethane Hydrochloride (commonly known as Tris HCl) is a widely used buffering agent in biochemical and molecular biology research. As the hydrochloride salt of Tris, it provides enhanced solubility and stable buffering capacity, making it essential in laboratories worldwide.
Basic Information
Chemical Name: Tris(hydroxymethyl)aminomethane Hydrochloride
CAS No.: 1185-53-1
Molecular Formula: C4H12ClNO3
Molecular Weight: 157.6 g/mol
Appearance: White crystalline powder
pKa (of Tris base): ~8.1 at 25°C
Buffering Range: pH 7.0 – 9.0
Key Features
Excellent pH Stability – Maintains consistent pH in biological and biochemical experiments.
High Solubility – Easily dissolves in aqueous solutions for quick preparation.
Versatile Applications – Compatible with a wide range of biological and chemical processes.
Reliable Performance – Ensures reproducibility in sensitive laboratory assays.
Applications in Research and Industry
Buffer Preparation:
Tris HCl is one of the most common components in biological buffer systems, widely used in molecular biology, biochemistry, and microbiology.
Electrophoresis:
It is a key ingredient in buffer systems for SDS-PAGE and nucleic acid electrophoresis, ensuring sharp separation of proteins and DNA.
Cell Culture and Diagnostics:
Tris HCl stabilizes pH in various media and diagnostic formulations.
Enzyme Reactions:
Provides an optimal environment for enzymatic activity by maintaining stable pH.
Pharmaceutical and Biotechnology:
Used in drug formulations, protein purification, and nucleic acid research due to its biocompatibility.
Market and Supply
The demand for Tris HCl (CAS 1185-53-1) continues to grow in the life sciences, pharmaceutical, and biotechnology sectors. Manufacturers supply it in different grades such as laboratory grade, molecular biology grade, and GMP-compliant pharmaceutical grade. Reliable sourcing with complete quality documentation (COA, MSDS, TDS) is essential for research and production.
Safety and Handling
Store in a tightly closed container in a cool, dry place.
Avoid inhalation of dust and direct skin or eye contact.
Use standard laboratory protective equipment.
Conclusion
Tris(hydroxymethyl)aminomethane Hydrochloride (1185-53-1) is a fundamental buffering agent for life science research, diagnostics, and pharmaceutical manufacturing. With its broad buffering range, high solubility, and reliable stability, it remains indispensable in laboratories and industries worldwide.
For laboratories and companies seeking high-purity Tris HCl, working with trusted suppliers ensures consistent quality and reliable delivery.