1,3-bis(tris(hydroxymethyl)methylamino)propane, commonly known as Bis-Tris Propane (CAS 64431-96-5), is a widely used buffer compound in biochemical and molecular biology applications. It belongs to a class of zwitterionic buffers and is highly valued for its stability, biocompatibility, and optimal performance in a wide range of laboratory experiments.
Basic Information
Chemical Name: 1,3-bis(tris(hydroxymethyl)methylamino)propane
CAS No.: 64431-96-5
Molecular Formula: C9H22N2O6
Molecular Weight: 266.29 g/mol
Appearance: White crystalline powder
pKa (of Bis-Tris Propane): ~6.5 at 25°C
Buffering Range: pH 6.0 – 8.0
Key Features of Bis-Tris Propane
Optimal pH Range: The pH buffering capacity of Bis-Tris Propane is ideal for a variety of biological applications, particularly in the slightly acidic to neutral range (pH 6.0 – 8.0).
Excellent Buffering Stability: It maintains a stable pH even under varying conditions, making it suitable for sensitive enzymatic assays.
Low Ionic Strength: Bis-Tris Propane has minimal ionic strength interference, ensuring that it won't affect the activity of proteins or enzymes in biological reactions.
Compatibility with Biological Systems: It is non-toxic and highly biocompatible, making it an excellent choice for use in cell culture media and other biological experiments.
Applications in Industry and Research
1. Electrophoresis
Bis-Tris Propane is commonly used in electrophoresis, especially in SDS-PAGE for protein separation. Its buffering properties allow for enhanced protein resolution, particularly in studies involving larger proteins or when working at neutral pH levels.
2. Protein Purification
In protein biochemistry, Bis-Tris Propane is used in buffer systems for protein purification. Its ability to maintain a stable pH ensures that proteins remain active and stable during purification processes, whether in chromatography or other separation techniques.
3. Enzyme Assays
The buffer is ideal for enzymatic assays, especially when enzymes are sensitive to pH variations. By providing a consistent pH environment, Bis-Tris Propane optimizes enzyme activity and improves the accuracy of experimental results.
4. Cell Culture and Biotechnology
In cell culture media and biotechnological applications, Bis-Tris Propane plays a key role in maintaining an optimal environment for cell growth and biochemical reactions. Its biocompatibility and ability to maintain physiological pH conditions make it an indispensable tool in many laboratory settings.
5. Molecular Biology
It is also used in the preparation of molecular biology buffers, such as those used in nucleic acid extraction and analysis, including PCR and RNA isolation.
Market and Supply
The demand for 1,3-bis(tris(hydroxymethyl)methylamino)propane has increased in the pharmaceutical, biotechnology, and research sectors due to its versatility and reliability. Suppliers typically offer Bis-Tris Propane in various grades, including laboratory-grade and GMP-grade formulations, to meet different application needs. Ensuring product consistency through proper certifications (e.g., COA, MSDS) is crucial for industries requiring high-quality reagents.
Safety and Handling
Handling: Bis-Tris Propane is generally considered safe when handled according to standard laboratory procedures.
Storage: Store in a cool, dry place away from strong acids or bases.
Precautions: Wear gloves and safety goggles to avoid direct contact with skin or eyes. Avoid inhalation of dust.
Conclusion
1,3-bis(tris(hydroxymethyl)methylamino)propane (CAS 64431-96-5), or Bis-Tris Propane, is a versatile and reliable buffer widely used in molecular biology, protein chemistry, and biotechnological applications. Its ability to maintain a stable pH, low ionic strength, and compatibility with biological systems makes it indispensable in laboratory experiments. For researchers and manufacturers seeking high-quality Bis-Tris Propane, partnering with reputable suppliers ensures consistent quality, documentation, and on-time delivery.