What is Tapso,Na?
Tapso,Na (N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid sodium salt, CAS 105140-25-8) is a zwitterionic buffering agent commonly used in biochemical and molecular biology research. It belongs to the Good's buffers family, which are well-known for their minimal interaction with biological processes and high stability under experimental conditions.
As the sodium salt form of TAPS buffer, Tapso,Na offers improved solubility and is widely applied in enzyme assays, protein research, and electrophoresis systems.
Key Properties of Tapso,Na
CAS Number: 105140-25-8
Molecular Formula: C7H16NO7SNa
Buffering Range (pKa): ~7.6 – 8.2
Appearance: White crystalline powder
Solubility: Highly soluble in water
Stability: Chemically stable, non-toxic, minimal absorbance in UV region
Applications of Tapso,Na
Biochemical Research
Used as a buffering agent in enzyme activity studies.
Maintains stable pH in protein purification and structural analysis.
Molecular Biology
Suitable for nucleic acid electrophoresis and DNA/RNA isolation protocols.
Provides stable conditions for hybridization reactions.
Cell Culture Studies
Helps maintain physiological pH in cell-based assays.
Minimizes interference with cellular metabolism.
Electrophoresis & Chromatography
Applied in electrophoretic separation systems.
Ensures reproducibility in chromatographic analysis.
Advantages of Tapso,Na
Narrow buffering range ideal for biological systems.
Low toxicity, safe for use in sensitive assays.
Compatible with most metal ions and biomolecules.
Minimal absorption at 260/280 nm, making it suitable for nucleic acid and protein research.
Why Choose Tapso,Na for Research?
Tapso,Na is valued for its reliability, reproducibility, and versatility in life sciences. Its buffering capacity in the near-neutral range makes it particularly useful for enzyme kinetics and protein biochemistry, where maintaining consistent pH is crucial.
With the increasing demand for high-purity biological buffers in pharmaceuticals, biotechnology, and academic research, Tapso,Na continues to play an essential role in laboratory and industrial applications.